TSB - Tryptone Soya Broth is a qualitative test for detection of microorganisms in blood. Sterile, in glass bottles.
Hi-Safe™Blood Culturing system is a fast, efficient and simpler detection and preliminary identification system for microorganisms from blood.
The liquid nutrient media are available in two sizes - for Paediatric use (20ml) for Adult use (70ml).
In most cases of bacteremia the organisms are not numerous, hence a relatively large amount of blood has to be used as inoculum. The blood’s natural bactericidal or bacteriostatic action as well as antibiotics used in therapy may delay or reduce the chances of obtaining a positive culture. Hence substances such as liquoid (Sodium polyanethol sulfonate, SPS) may be used as a non-toxic anticoagulant which enables bacterial growth and prevents the action of natural bacterial inhibitors of blood.
SPS inhibits the activity of streptomycin, polymyxin B, kanamycin and gentamycin. Van Haebler and Miles first demonstrated the usefulness of SPS in Blood Culture Medium.
All HiSafe™ Blood Culture media have been developed to support the growth of a variety of clinically significant pathogenic microorganisms and also fastidious ones. It offers a wide range of culture media like:
- BHI - Brain Heart Infusion, BHI - Supplemented w/ 0.05% SPS
- Columbia Broth
- Fluid Thioglycollate Medium w/0.05% SPS
- Glucose Broth Supplemented w/ 0.05 % SPS
- Hartley Broth, Hartley Broth w/ 0.05% SPS
- Modified Wilkins Chalgren Broth
- Schaedler Broth
- Thioglycollate Broth
- Tryptone Soya Broth
- Tryptone Soya Broth Supplemented w/ 0.05%SPS
- Tryptone Soya Broth w/ 10% Sucrose
Blood is one of the most important specimens received by the laboratory. Since blood is normally sterile, blood cultures are of great importance in diagnosing blood - stream related infections such a s endocarditis, typhoid fever, pneumonia, suppurative thrombophlebitis, infections of vascular graftsetc.
Procedure: Blood is collected from the patient (before commencing antibiotic therapy preferably) using asterile blood collection set and transferred aseptically to HiSafe™Blood Culture Bottle containing a suitable growth medium (depending on the suspected pathogen). The bottle is then incubated and observed for turbidity, colour change, hemolysis, gas production.