Without: Sodium bicarbonate
NCTC 109 Medium is one of the chemically defined medium in the series on NCTC media developed by Virginia Evans of the Tissue Culture Section of National Cancer Institute, Bethesda. NCTC 109 was the result of many years of development and modifications. The medium was originally formulated to establish and maintain a strain of mouse cells, L929. The medium has been shown to support growth of fibroblast-like and epithelial- like cells of both normal and malignant origin from mice, hamsters, monkeys and humans.
NCTC 135 is similar to NCTC 109 except that L-Cysteine has been replaced with L-Cystine due to possible side effects of L-Cysteine on certain cell lines.
AT138 is NCTC 109 Medium with L-glutamine. Its does not contain Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.