Anti-Coli A is a polyspecific screening reagent that is intended for use in serological detection of specific antigens of E.coli strains.
- Method: Slide agglutination. If the isolated strain has E.coli antigen, within the range of the testing reagent, antigen-antibody reaction results in a visible agglutination. A negative result is reported in the absence of agglutination.
- Range: polyspecific (O 1, O 2, O 18, O 78); serological detection of O 1-, O 2-, O 18 or O 78 antigen.
- Composition: A mixture of monoclonal antibodies derived from the supernatant of the culture medium of hybridoma cell lines secreting antibodies against the specific E.coli antigens.
- Preservative: Sodium Azide (NaN3) 0.9mg/ml
- Form of the reagent: Liquid
- Storage: 2-8⁰C
An isolate with positive results requires testing with monospecific reagents for further characterisation and to rule out unspecific agglutinations a check by the Gruber-Widal test (confirmation test).
Anti-Coli A is relevant to veterinary medicine by E.coli diagnostics in young poutry.