RIDA®QUICK Histamin is a colorimetric assay for the quantitative analysis of histamine in fish meal. If fresh fish samples are to be analyzed a validation has to be carried out (employ histamine-free and histamine containing (spiked) samples for each fish species).
All reagents required for the enzyme immunoassay - including standards - are contained in the test kit.
For in vitro use only.
Histamine is one of the biogenic amines. The amount of histamine formed depends on the bacterial species, the temperature and time of exposure, and may exceed 1000 ppm (mg/kg). If concentrations of amines exceed normal levels, possibly due to bacterial contamination of food, harmful effects may occur. The contents of histamine in food is regarded as criterion of quality.
Test Format: Microtiter plate 96 wells non-coated (12 strips with 8 removable wells each) and 6 x 8 Ion exchanger columns
Standard Range: 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm, 40 ppm
Sample Preparation: extraction, clean-up with an ion-exchange column
Incubation Time: approx. 10 min
- Fresh fish: 20 ppm
- Fish meal: 125 ppm (coresponding to the standard substance)
- Histamine: 100%
- L-Histidine: < 1%
- Tyramine: < 1%
- L-Tyrosine: < 1%
- L-Phenylalanine: n.d.
- L-Tryptophan: n.d.
- Non-aromatic amino acids: n.d.
- Tryptamine: n.d.
- Serotonine: n.d.
YouTube - Allergen analysis test procedure for RIDASCREEN® ELISA