SureFast® Salmonella ONE is a real-time PCR kit for the fast and simple isolation and detection of Salmonella DNA from enrichment cultures. Each reaction contains an internal amplification control (PLUS).
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions in the FAM and VIC/HEX channel at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen RotorGene Q and Cepheid SmartCycler®.
Test Format: 100 reactions
Sample Preparation: Thermal lysis without additional steps
Detection Limit: ≤ 5 DNA copies, 1 cfu after enrichment