SureFast® Listeria monocytogenes PLUS is a real-time PCR kit for the direct, qualitative detection of DNA sequences of the Listeria monocytogenes virulence-associated gene prfA. Each reaction contains an internal amplification control (PLUS).
For DNA-preparation the use of SureFast® PREP Bacteria.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.02, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
Test Format: 100 reactions
Detection Limit: ≤ 5 DNA copies, 1 cfu after enrichment