With: 15 mM HEPES buffer and Trace elements
Without: L - Glutamine and Sodium bicarbonate
Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12 1:1 Mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.
AT139A is DMEM / Nutrient Mixture F-12 Ham with HEPES buffer and trace elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It does not contain L - Glutamine and Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.