With: L - Glutamine and 15 mM HEPES buffer
Without: Trace elements and Sodium bicarbonate
Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12 1:1 Mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.
AT140 is DMEM/Nutrient Mixture F-12 Ham with L - Glutamine and HEPES buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It does not contain trace elements and Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.