With: L-Glutamine
Without: Tryptose Phosphate broth and Sodium bicarbonate
Glasgow’s Minimum Essential Medium is a modification of Basal Medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells for investigating the genetic factors affecting cell competence.
AT102 is Glasgow's Minimum Essential Medium with L-glutamine. It does not contain tryptose phosphate broth and Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.