With: L-Glutamine
Without: HEPES buffer and Sodium bicarbonate
Iscove’s Modified Dulbecco’s Medium is an enriched modification of Dulbecco’s Modified Eagle’s Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions.
AT160 is Iscove's Modified Dulbecco's Medium with L-glutamine. It does not contain HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.