SureFast® Norovirus PLUS is a real-time PCR kit for the direct and qualitative detection of norovirus.
The test contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC/HEX) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Applied Biosystems 7500 and Qiagen RotorGene Q.
Test Format: 100 reactions
Detection Limit: ≤ 25 RNA copies