With: Earle’s salts and NEAA

Without: L-Glutamine and Sodium bicarbonate

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hanks salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures.

AT017А is Minimum Essential Medium Eagle with Earle’s salts and NEAA and is modified for autoclaving. Autoclavable media offer a convenient alternative to membrane sterilized liquid medium. It is modified to include heat stable components to ensure that product efficacy is maintained after autoclaving. L-glutamine is heat labile, hence has been omitted from the formulation. It does not contaun Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.