SureFast® Influenza A H5/H7/H9 4plex is a multiplex real-time PCR kit for the direct, qualitative detection and differentiation test of the influenza A-subtypes H5, H7 and H9.
For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II/Cobast and Applied Biosystems 7500.
Test Format: 100 reactions
Detection Limit: ≤ 25 RNA copies