With: L-Glutamine and 25mM HEPES buffer

Without: Sodium bicarbonate

Ham’s Nutrient mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham’s nutrient mixture F-10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues.

AT083 is Nutrient Mixture F-10 Ham with L-glutamine and 25mM HEPES buffer. HEPES a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Its does not contain Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.