With: L-Glutamine

Without: Sodium bicarbonate

Ham’s Nutrient mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham’s nutrient mixture F-10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues.

Kaighn's modification of Ham's F-12 is a complex formulation of F-12 with increased amounts of amino acids and pyruvate. Salts used in this formulation are as given by Konisberg. This modification favors the growth and differentiation of rat and chicken cells and primary human liver cells.

AT106 is Nutrient Mixture F-12 Ham with L-glutamine. Its does not contain Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.