Without: L-Glutamine and Sodium bicarbonate

Ham’s Nutrient mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham’s nutrient mixture F-10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues.

Ham's Nutrient Mixture F12, Coon's modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with Sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid.

AT146A is Coon's modification of Nutrient Mixture F-12 Ham without L-glutamine and Sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.