With: L-Glutamine
Without: Sodium bicarbonate
Waymouth Medium MB 752/1 was developed in 1959 for the cultivation of mouse L cells, NCTC clone 929. Waymouth's medium is a comparatively simple medium with lesser ingredients than its other synthetic counterparts. The medium comprises of amino acids, vitamins, the purine base hypoxanthine, salts, and glucose. This medium can be used for culturing many fastidious cell lines and also whole organ culture.
AT091 is Waymouth Medium MB 752/1 with Lglutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.