AFLAOCHRA PREP® are multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2 and ochratoxin A in wheat, barley, maize, paprika, black pepper, dried fruit including sultanas, raisins, figs and apricots, in conjunction with HPLC or LC-MS/MS.

General Information:

Mycotoxins are toxic metabolites produced by molds. They can have serious acute and chronic effects on the health of humans as well as animals. Mycotoxin analysis of food and feed is therefore necessary and often required by legislation.

Test Principle:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.

Test Format: 50 narrow format immunoaffinity columns (1 mL)

Analytical yield: 70-110%