SureFast® Clostridium botulinum Screening PLUS is a real-time PCR kit for the detection of DNA of the Botulinum neurotoxins (BoNT) A, B, E and F of Clostridium botulinum, Clostridium baratii and Clostridium butyricum. Each reaction contains an internal amplification control (PLUS).
For DNA-preparation the use of SureFast® PREP Bacteria is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.02, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
Test Format: 100 reactions
Detection Limit: ≤ 50 DNA copies