The Real Time PCR analysis system is the latest generation of Tianlong Technology, tailored to meet the experimental needs of high-end users. This product has many advantages such as scientific and efficient temperature control system and optoelectronic system, powerful and easy-to-use software analysis functions, and humanized control methods. It is easy to implement downstream multiple gene detection, quantitative analysis, SNP analysis, HRM analysis, and other applications.
- 4-channel synchronous detection
Four kinds of conventional excitation and detection channels, compatible with most fluorescent dyes and probe types, enabling detection of absolute quantification, relative quantification, and genotyping; The need for high-sensitivity detection makes detection more convenient, professional and accurate.
- Diversified operation methods
Based on the one-to-one control of the classic external computer, it has creatively introduced the remote operation of the local area network and local operation mode of the instrument. The built-in 10.4-inch touch screen is equipped with self-developed control software, experimental settings, real-time monitoring of experiments, and instrument settings.
- High sensitive and precise optical system
The fluorescence excitation light source adopts the bright long-life LED, and the fluorescence detection system is maintenance free. The optical system is located on the upper part of the instrument. During operation, the top is excited and scanned. There is no need to worry about the adverse effects of dust in the vent post. The compact and scientific and logical optical system integrates four fluorescence detection channels to achieve a number of technological breakthroughs. At the same time, it increases the temperature control to ensure the accuracy and stability of fluorescence detection. It takes only 7 seconds to complete a one-by-one scan of 96 channels of 4 fluorescence channels, with high efficiency and no fluorescence edge effect.
- Accurate and efficient temperature control system
Based on the Peltier effect, six semiconductor cooling sheets are arranged under the Block. The temperature uniformity, accuracy, and temperature rise/fall rate are all significantly improved, shortening the experimental period, realising the temperature gradient function, eliminating the need for annealing temperatures in the past. Repeatedly explored to improve the efficiency of the experiment.
The product has a variety of advanced technologies: WINDOWS operating system, color display screen, dual modules operate independently, a variety of interchangeable modules, computer on-line function, print function, large data storage capacity and expansion capabilities, etc.; function of the qualitative PCR product perfect to the extreme, powerful features will meet the higher requirements of an experiment.
Specification:
| Sample capacity(wells): | 96 |
| Reaction volume: | 0-100 μL |
| Consumables: |
0.2ml 96-well plates, 0.2ml strips, 0.2ml PCR tube |
| Temperature range: | 0°C to 100°C |
| Max heating rate: | 6.1°C/sec |
| Average heating rate: | 4.5°C/sec |
| Max cooling rate: | 5.0°C/sec |
| Average cooling rate: | 2.8°C/sec |
| Temperature accuracy: | ±0.1°C |
| Temperature uniformity: | ±0.1°C |
| Gradient range: | 1°C - 40°C |
| Gradient block: | 12 row |
| Special temp. protocol: | Gradient PCR, Long PCR, Touch Dcmn PCR |
| Temperature range: | Room Temperature - 110°C |
| Excitation source: | 4LEDs |
| Detector: | Photodiode |
| Detection position: | Excitation and scan at the top |
| Detection method: |
4 channels scanning at the same time: 1. 465 /510 (FAM, SYBR Green l, SYTO9, EvaGreen, LC Green) 2. 527/ 563 (HEX, VIC, TET, JOE) 3. 580 /616 (ROX, Texas Red) 4. 632/ 664 (Cy5) |
| Detection time: | 7 seconds for 96 wells for all channels |
| Fluorescence linearity: | r ≥ 0.990 |
| Fluorescence dynamic range: | Adjustable |
| Sample linearity: | r ≥ 0.999 |
| Sample repeatability: | Ct value CV≤0.5% |
| Sample dynamic range: | 1-10 copies |
| Data storage: | Upload and download through USB disk |
| Power failure protection: | Automatically start running experiments after the power supply |
| Admin.management: | The administrator can set functions limits for users |
| LIS connection: | CSV, Excel, TXT format data output, Оpen port for LIS connection |
| PC operating system: | Win 7, Win 10 |
| Communication port: | 1 ethernet and 3 USB |
| Dimension: | 35.5 x 48 x 48.5 cm |
| Power supply: | 200 to 240V |
| Weight: | 30 kg |
| Work environment: | Temperature: 10°C-30°C Humidity: 20%-85% |
